is one of the basic culture media that is used without enrichment for the cultivation and isolation of a number of organisms. By adding 5% of horse or sheep blood serum and enriching it, we enable the growth of hard-to-grow bacteria and check their hemolytic activity. If you add 7% blood without fibrin at a temperature of 70 degrees and keep the temperature constant for 10 minutes, Chocolate Agar medium is obtained. To incubate the given culture medium for 18 to 48 hours at a temperature of 35-37.