What is the culture medium?
Culture medium or growth medium is a liquid or gel designed for the growth of microorganisms, cells or small plants. Cell culture medium is generally a good source of energy and compounds that regulate the cell cycle. A normal culture medium contains various compounds such as amino acids, vitamins, mineral salts, glucose, serum and hormones for the growth of organisms.
What is natural culture medium? Natural culture mediums are biological fluids that are very useful for the growth of a wide range of animal cells. These environments are not reproducible due to the unknown composition of the media, and this is one of the disadvantages of using them in cell culture. Types of natural culture media include biological fluids, tissue extracts, and plasma.
What is artificial culture media? Artificial (synthetic) culture media consists of combining nutrients (organic and inorganic materials) including vitamins, salts, and oxygen gases. And carbon dioxide, serum proteins, carbohydrates and cofactors are formed. Different artificial culture environments have been made for different purposes, including the following:
What types of culture medium are based on physical state?
Based on their consistency and physical state, culture mediums are divided into three main categories: solid, semi-solid, and liquid, which are used for special applications such as identifying and diagnosing microorganisms, examining changes in strains in the growth medium, examining the movement of microorganisms, and performing Biochemical tests are used in diagnostic and research laboratories.
Solid Culture Media
"Solid Culture Media" is used to identify and diagnose many bacteria and pathogens. It works. Also, this type of environment is used to observe and check the appearance of bacterial colonies and check the changes during cultivation. These types of media usually contain 1 to 2% agar and are mainly made in Petri dishes. They are also used for deep cultivation inside test tubes. Examples of this include nutrient agar and blood agar.
What is a semisolid culture medium?
Semisolid culture media is made by adding 0/ 2 to 0.5% of agar is prepared into liquid medium and it is mainly used as transfer medium and strain motility check. For example, "Cary-Blair" is one of the types of semi-solid media. It is not added and is used in preparing enriched environments and performing biochemical tests. The growth rate of bacteria in this type of growth environment can be seen by checking the turbidity of the solution.